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Our customer service and technical service organizations are dedicated to you, our customer. Technical Information. hJG1xtA8TB2lkD,44.`l"TP6bw12 ckf+Op02`X siC$5 [#dx>002_s*05XN`pr2`e dfT~iC0 650aw`X t 13 jtXE rZcI bg.N(@ c endstream endobj 69 0 obj <>>> endobj 70 0 obj >/PageTransformationMatrixList<0[1.0 0.0 0.0 1.0 0.0 0.0]>>/PageUIDList<0 1213>>/PageWidthList<0 594.0>>>>>>/Resources<>/ExtGState<>/Font<>/ProcSet[/PDF/Text/ImageC]/XObject<>>>/Rotate 0/TrimBox[0.0 0.0 594.0 792.0]/Type/Page>> endobj 71 0 obj <> endobj 72 0 obj [/ICCBased 114 0 R] endobj 73 0 obj [/ICCBased 100 0 R] endobj 74 0 obj <>stream Millipore Pellicon 3 Cassettes with Ultracel. Primary and secondary antibodies, blockers and custom services. Use a few drops of suitable lubricant on the tie-rods and between the nuts and the washers if required. Advanced, high-performance cassettes ideal for todays higher titer therapeutic antibodies as well as the more demanding filtration processes require higher operating pressures, temperatures and caustic cleaning regimes.Pellicon 3 cassettes with Ultracel membranes are the optimum tangential flow filtration devices for the filtration of monoclonal antibodies and other therapeutic proteins.Pellicon 3 cassettes with Biomax membranes are the optimum tangential flow filtration devices for the filtration of solutions containing albumin, hormones, vaccines and growth factors. Are you sure you want to delete your template? Pellicon 3 Cassettes - Pellicon 3 Cassettes - Merck If depyrogenation is required, it should only be performed after the system has been cleaned, sanitized, and flushed. Pioneering excellence with Steritest Symbio Pumps. Ncrfhgy epcrhtcs hs Fmaamperc\mnfh mg t`c S.\. It is not intended to provide validation protocols or supporting data for validation purposes. Sanitization agents must comply with applicable local regulations. User Guides. Installation Three Pellicon 3 cassettes with 0.11 m2 of Ultracel 30 kDa mem-brane were individually installed in a holder and tightened with a cali-brated torque wrench at 180 in-lbs. This P3C010C10 Pellicon 3 ultrafiltration device features a pore size of 10 kDa NMWCO, a filtration area of 1.14 m 2, a PLCGC membrane, and dimensions . Overview. Optimal cross flow will depend on the actual solution being filtered. Filters may be stored in holders with the feed, retentate and filtrate isolation valves closed and enough compression on the cassettes to ensure that the storage fluid cannot escape or evaporate. The Power Switch on the Hydraulic Control Unit HS-1 must be in the ON position, and the green Power Indicator must be illuminated. H\Qk@>soHm Q)-;:7\f&w6pMpplYk9ce deep hex socket supplied with the Pellicon Cassette Holder. Check for dirt or corrosion on tie rods or bolts, Remove old tape, re- tape adequately with PTFE tape, Missing O-ring or ferrule on fittings of defective sanitary gasket, Replace missing component, gasket, or entire fitting, Replace clamp with smaller diameter clamp, Disassemble stack, remove stack and check for debris, See remedy under Tube/Fitting Leaks above, Bubbling at external surface of filter stack, Dirt, debris or loose holder, or chemical incompatibility. Loosen and remove the hand wheels on the clamp rods at the opposite end plate (of the same holder level). This guide provides installation and operation procedures for Pellicon 2 cassettes. Storage solution must be introduced into the system through the cassettes and the system piping to put the system in a bacteriostatic state in between process runs. 0000433950 00000 n Manifold adapter plate for Pellicon 3 cassette manual process-scale holder and benchtop holder installation. 80 psi TMP at 4-40C; 40 psi TMP at 4-50C. Slide the end plate onto the tie rods and press the end plate against the cassette. RUN mode is required for process operations, including flushing and cleaning. It is recommended that a set of spare parts be kept on hand at all times. What is the part number for the Gauge Kit that is used with the Pellicon XL? Pellicon 3 88 cm2 and 0.11 m2 Cassette Holder Installation. 0000016059 00000 n 0000453522 00000 n *1Ilpu@P}X(+0fX:FqK3*vv0\;rCap*.uZ\(8!m=z\89^w&INO3hk]Q}_,GY/@OzZhhWt1%lLv:/;9T;?;M/%~?Wzqd/u|D.^L~Ko gz Pellicon 3 Cassette with Biomax Membrane NMWCO 30 kDa | Sigma-Aldrich socket supplied with the Pellicon Cassette Holder. The Pellicon 3 Cassette with Biomax Membrane is perfect for the filtration of monoclonal antibodies and solutions containing albumin, hormones, vaccines and growth factors. Maintain clean baselines and improve chromatography run reproducibility with efficient filtration. The filters may also be removed from the system and placed in sealed bags or containers. purification system (90 pages), Manual will be automatically added to "My Manuals", Connecting Pressure Gauge Adapter Fittings to FEED and RETENTATE Fittings, Assembling the Pellicon 2 and 3 Mini Holder, Water Filtration Systems MILLIPORE RIOS 3 User Manual, Water Filtration Systems Millipore Milli-Q Plus Installation And Operation Manual, Water Filtration Systems MILLIPORE Simplicity Operating And Maintenance Manual, Water Filtration Systems Millipore Synergy User Manual, Water Filtration Systems Millipore direct-q 3 R User Manual, Water Filtration Systems Millipore Milli-Q User Manual, Water Filtration Systems Millipore Milli-Q Advantage A10 User Manual, Water Filtration Systems Millipore Synergy R User Manual, Water Filtration Systems Millipore Elix Essential 3 User Manual, Water Filtration Systems Millipore Milli-DI Instruction Manual, Water Filtration Systems Millipore Milli-Q CLX 7040 User Manual, Water Filtration Systems Millipore Milli-Q Integral 3 User Manual, Water Filtration Systems Millipore Elix 20 User Manual, Water Filtration Systems Millipore Milli-Q Direct 8 System User Manual, Page 7: Assembling The Pellicon 2 And 3 Mini Holder, Page 8: Connecting Pressure Gauge Adapter Fittings To Feed And Retentate Fittings. Turn the knurled locking collar counterclockwise to lock. 0000003603 00000 n Install the tie rod spacers, washers and nuts. Set the torque wrench by turning the knurled locking collar clockwise or by pulling the handle out to unlock. NOTE Pellicon 3 88 cm2 and 0.11 m2 Cassettes must be installed in a Pellicon Cassette Holder, Catalog Number XX42PMINI. All references to Merck refer to Merck KGaA, Darmstadt, Germany. Water QualityReverse osmosis (RO) water or water for injection (WFI) is recommended. Align the filter cut-outs with the tie rods, and insert the filter so it is parallel with the manifold as, shown in Figure 8. Turn on the feed pump and pump water into the feed port of the Pellicon holder. Optimize your QC lab workflow and ensure regulatory compliance. JY-x\8|hnR6~V9* ,?C;y6J6h[8sMZYRm[ [YB vOjtnAJQk7 zw When the wrench clicks, this indicates that the set force has been reached. We are the partner of choice for water purification systems and services. Several studies have been included to provide you with a well-rounded overview of the entire Fully open the retentate valve (V4) (and permeate valve (V5) if present) and direct both the retentate and permeate to drain. 0000015807 00000 n 0000463159 00000 n Training programs and seminars designed for you. Fill the tank with the cleaning solution. Pellicon 3 Cassettes - Millipore - Yumpu When a Pellicon Holder is ordered, what do I receive? Applications Products Services Support. 0000003639 00000 n Review chemical compatibility of filter. *Use milder conditions if effectiveness of agent is still observed. In-process controls are used to assure stability of the process. Millipore Sigma Pellicon 3 Cassettes Installation And User Manual 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 page of 16 Go Table of Contents Troubleshooting Bookmarks Advertisement Quick Links x Download this manual 4 Manual Process-Scale Holder Installation 7 Flushing and Cleaning 8 Cleaning Installation and User Turn on the feed pump and pump water into the feed port of the Pellicon holder. Up to ten 1.14 m2 cassettes may be installed per side if required. Learn More >> Pellicon 3 Cassettes Advanced, high performance cassettes ideally suited to today's demanding filtration processes that require . &T](l%1m%Wwv&RK=\C/qpzqdJVB bm@;,=d,C^}`V,B Greater control & increased flexibility for both small & large molecule development & manufacturing processes. However, a chemical sanitization can be done; refer to the user guide for recommendations. On the first holder level (if there are more than one), remove the TC clamps and inserts holding the hydraulic cylinders to the clamp rods. endstream endobj 259 0 obj <>stream 0000434020 00000 n Trust in one of the most highly published & validated portfolios of antibodies. Create the perfect solution - match and clear hexagones. Our portfolio of innovative products, services and solutions. Industrial Microbiology. Pellicon 3 88 cm 2 cassette filter holder; Ultrafiltration for Biopharmaceutical Manufacturing; Therapy Production; Buy online from Sigma Aldrich. Pellicon 3 Cassettes must be operated while under compression from the holder assembly to ensure that the cassettes will seal properly. This guide provides installation and maintenance procedures for Pellicon 3 cassettes. Merck KGaA, Darmstadt, Germany, 2014. Torque and Compression Force of Pellicon 2 and 3 Cassettes - EMD Millipore Pellicon3 Cassettes - Installation and User Guide. Required fields are marked *. Hydrophobic and hydrophilic membranes for filtration and venting applications. Record the feed and permeate flow rate, feed, retentate, and permeate pressures, and the temperature of the water. System and cassette not completely flushed, System and cassette not completely cleaned, Check torque procedure, retorque. Hold-up Volume. Tighten the nuts using the torque wrench and 15/16 in. 80 psi (5.5 bar) TMP @ 40C (initial use), 80 psi (5.5 bar) TMP @ 40 C (initial use), 30 psi (2 bar) @ 25 C ) @ 25C for 24 hours. Replace any damaged components. 11. AN1065EN00 Rev C.indd | Manualzz ',Av]g_M&Ug3pZe3Op%]!i@_#(%F7~=0|:K )RBw_/G}nP%igyuWtXU#7{

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